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J&J Family of Companies Associate Scientist I, CAR Screening in Spring House, Pennsylvania

Associate Scientist I, CAR Screening - 2306122220W


Janssen Research & Development, L.L.C., a division of Johnson & Johnson's Family of Companies is recruiting for an Associate Scientist I, CAR Screening located in Spring House, PA.

At the Janssen Pharmaceutical Companies of Johnson & Johnson, we are working to create a world without disease. Transforming lives by finding new and better ways to prevent, intercept, treat and cure disease inspires us. We bring together the best minds and pursue the most promising science. We are Janssen. We collaborate with the world for the health of everyone in it. Learn more at www.janssen.com and follow us @JanssenGlobal. Janssen Research & Development, LLC is part of the Janssen Pharmaceutical Companies.

The Cell Therapy team at Janssen Therapeutics Discovery is seeking a highly motivated lab-based Associate Scientist to join our Chimeric Antigen Receptor (CAR) Screening and Discovery team. The Associate Scientist I will join a cross-functional group of experts building a high throughput CAR screening pipeline for autologous and iPSC-derived effector cell types.

The ideal candidate is an organized, self-driven scientist that thrives in a collaborative team-based environment, is excited to learn and has experience setting up in vitro functional assays to measure T cell function using both representative cell lines and primary human cells. Key activities will include performing in-vitro functional assays for large scale screening of CAR candidates, immune cell phenotyping through multi-color flow cytometry & cell line maintenance. Any experience in immunology, cell therapy and/or immuno-oncology is a plus but not required.

Come join us and become part of a dynamic team working to achieve curative responses in patients with cancer by building best-in-class cell therapies!

Key Responsibilities:

  • Design and complete experiments based on protocols.

  • Analyze and interpret experimental data.

  • Thaw, culture and maintain a variety of mammalian cell lines using aseptic technique.

  • Complete multi color immune cell phenotyping and FACS analysis.

  • Assist in CAR T cell generation, expansion, characterization, and cryopreservation.

  • Coordinate experimental activities with other team members to deliver results that meet pre-defined timelines.

  • Maintain a detailed, accurate, complete, and up-to-date laboratory notebook

  • Data generation, analysis, maintenance, and communication to immediate team and cross-functional teams.



  • Bachelor's degree in Life Sciences (e.g. Biology, Biotechnology) or a related field with 1-2 years of academic and/or industry experience is required.

Experience and Skills:

  • Experience with in vitro cell culture: aseptic technique, cell-line culturing, freezing and thaw is required.

  • Experience with multi-color flow cytometry (3-5 colors) and downstream data processing/analysis (FlowJo) is preferred.

  • Experience with functional characterization such as cytotoxicity assays, proliferation and cytokine measurements is preferred.

  • Experience working with immune cell types such as murine or human T cells, NK cells or Gamma Delta T cells is a plus.

  • Willingness to think creatively, be flexible and able to work in a fast paced, changing environment is highly desired.

  • Ability to multitask, independently organize time, and plan specifics of work is desired.

  • Detail oriented, with excellent verbal and written communication is highly desired.

  • Excellent record keeping and documentation skills as well as experience using ELN systems is a plus.

Johnson & Johnson is an Affirmative Action and Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, age, national origin, or protected veteran status and will not be discriminated against on the basis of disability.

For more information on how we support the whole health of our employees throughout their wellness, career and life journey, please visit www.careers.jnj.com.

Primary Location NA-US-Pennsylvania-Spring House

Organization Janssen Research & Development, LLC (6084)

Job Function R&D

Req ID: 2306122220W